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Biological response of osteoblast‐like UMR‐106 cells to the modified PHBV matrix—Effects of porosity and collagen dip coating
Author(s) -
Liu Hui,
Raghavan Dharmaraj,
Melaku Samuel,
Stubbs John
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32427
Subject(s) - materials science , porosity , osteoblast , coating , matrix (chemical analysis) , tissue engineering , scaffold , calcium , cell culture , chemical engineering , biomedical engineering , in vitro , composite material , chemistry , biochemistry , medicine , metallurgy , genetics , biology , engineering
In this study, we related porosity and collagen coating of poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) (PHBV) scaffold to the degree of cell proliferation on the engineered PHBV scaffold. Based on the [3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2h‐tetrazolium, inner salt] (MTS) assay, we established that UMR‐106 cell proliferation is maximum in collagen‐coated porous PHBV film followed by porous PHBV film and least in nonporous PHBV film. RT‐PCR analysis of the proliferated cells on tissue culture polystyrene (TCPS) and porous and nonporous PHBV scaffolds revealed that the proliferated cells retained their osteoblastic phenotype characteristics. Atomic absorption analysis was performed to measure the extent of calcium conversion by the cells grown on PHBV and TCPS. The calcium content of the culture media was used to indirectly measure the mineralization ability of the cells. The extent of calcium conversion by the cells was found to depend on the incubation time. Based on the results of the study, modified PHBV matrix seems to be a suitable matrix candidate for bone tissue engineering application. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010