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Long‐term cell‐mediated protein release from calcium phosphate ceramics
Author(s) -
Wernike Ellen,
Hofstetter Willy,
Liu Yuelian,
Wu Gang,
Sebald HansJörg,
Wismeijer Daniel,
Hunziker Ernst B.,
Siebenrock KlausArno,
Klenke Frank M.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32411
Subject(s) - coprecipitation , bovine serum albumin , protein adsorption , materials science , kinetics , bioavailability , calcium , phosphate , biophysics , adsorption , biochemistry , chemistry , pharmacology , biology , inorganic chemistry , organic chemistry , metallurgy , physics , quantum mechanics
Abstract Efficient delivery of growth factors from carrier biomaterials depends critically on the release kinetics of the proteins that constitute the carrier. Immobilizing growth factors to calcium phosphate ceramics has been attempted by direct adsorption and usually resulted in a rapid and passive release of the superficially adherent proteins. The insufficient retention of growth factors limited their bioavailability and their efficacy in the treatment of bone regeneration. In this study, a coprecipitation technique of proteins and calcium phosphate was employed to modify the delivery of proteins from biphasic calcium phosphate (BCP) ceramics. To this end, tritium‐labeled bovine serum albumin ([ 3 H]BSA) was utilized as a model protein to analyze the coprecipitation efficacy and the release kinetics of the protein from the carrier material. Conventional adsorption of [ 3 H]BSA resulted in a rapid and passive release of the protein from BCP ceramics, whereas the coprecipitation technique effectively prevented the burst release of [ 3 H]BSA. Further analysis of the in vitro kinetics demonstrated a sustained, cell‐mediated release of coprecipitated [ 3 H]BSA from BCP ceramics induced by resorbing osteoclasts. The coprecipitation technique described herein, achieved a physiologic‐like protein release, by incorporating [ 3 H]BSA into its respective carriers, rendering it a promising tool in growth factor delivery for bone healing. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010