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Chondrogenic differentiation of mesenchymal stem cells embedded in a scaffold by long‐term release of TGF‐β3 complexed with chondroitin sulfate
Author(s) -
Park Ji Sun,
Yang Hyun Jung,
Woo Dae Gyun,
Yang Han Na,
Na Kun,
Park KeunHong
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32388
Subject(s) - chondrogenesis , mesenchymal stem cell , materials science , chondroitin sulfate , scaffold , cartilage , biomedical engineering , tissue engineering , cartilage oligomeric matrix protein , microbiology and biotechnology , chemistry , glycosaminoglycan , biology , anatomy , pathology , biochemistry , osteoarthritis , medicine , alternative medicine
In this study, mesenchymal stem cells (MSCs) embedded in biodegradable and water‐swollen, elastic block copolymer scaffolds were assessed for MSC chondrogenesis. To determine the optimal conditions for chondrogenesis of the embedded rMSCs, transforming growth factor‐β3 (TGF‐β3) was physically conjugated with chondroitin sulfate (CS) and mixed into scaffolds, which were subsequently evaluated for the differentiation of transplanted rMSCs. In determination of CS‐bound growth factors for chondrogenesis, scaffold mixed with rMSCs and TGF‐β3 was then tested by growth factor release profiles, confocal laser microscopy, RT‐PCR analysis, real time‐QPCR, and histology. The results of several different analyses of the transplanted rMSCs embedded in the scaffolds showed that rMSCs coupled with a CS‐bound TGF‐β3 encapsulated scaffold evidenced superior cartilage tissue formation as measured by an assay of specific gene and protein expression. Moreover, the scaffold exhibited more rapid and more distinct morphology of differentiated rMSCs than was observed with other scaffolds, as determined by histology and immunochemical histology analysis. These results indicate that the elastic block copolymer scaffolds combined with a CS‐bound TGF‐β3 should prove very suitable matrix for cell‐based cartilage tissue engineering. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010