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Biofunctionalization of dispense‐plotted hydroxyapatite scaffolds with peptides: Quantification and cellular response
Author(s) -
Detsch Rainer,
Dieser Irina,
Deisinger Ulrike,
Uhl Franziska,
Hamisch Sabine,
Ziegler Günter,
Lipps Georg
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32386
Subject(s) - materials science , scaffold , covalent bond , peptide , stromal cell , cell adhesion , biomedical engineering , adhesion , biophysics , ceramic , fluorescence , composite material , biochemistry , chemistry , physics , organic chemistry , pathology , quantum mechanics , biology , medicine
Hydroxyapatite (HA) ceramic is a widely used synthetic bone substitute material for the regeneration of bone defects. We manufactured HA scaffolds with adjustable pore sizes and pore geometry by dispense‐plotting. In addition, we attached peptides covalently onto the HA surface and are able to simultaneously quantify the amount of covalently attached and adsorbed peptide down to the picomolar range with a novel fluorescence‐based detection method. In cell culture assays with stromal bone marrow cells, we observed a positive effect of biofunctionalization on cell differentiation after 21 days of culture when comparing the scaffold functionalized with the RGD motif containing adhesion peptide to an unmodified scaffold. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010