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Enhanced insulin production from murine islet beta cells incubated on poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyhexanoate)
Author(s) -
Yang XiaoDi,
Li HongMing,
Chen Miao,
Zou XiangHui,
Zhu LinYun,
Wei Chiju,
Chen GuoQiang
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32379
Subject(s) - materials science , islet , insulin , beta (programming language) , production (economics) , beta cell , endocrinology , biology , computer science , macroeconomics , economics , programming language
Islet transplantation represents an important alternative for the treatment of diabetes. However, the selection of suitable materials is critical for the success of such an implantation application. In this study, cellular migration, aggregation, and insulin production of a murine islet beta‐cell line, NIT‐1 cells on microbially produced polyesters poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyhexanoate) (PHBHHx), poly(3‐hydroxybutyrate‐ co ‐4‐hydroxybutyrate) (P3HB4HB) or polylactic acid (PLA) films were investigated. Spherical islet‐like structures were only detected on PHBHHx films after 48 h cultivation. To understand the mechanism underlying the formation of cell aggregates, NIT‐1‐GFP, a stable transfectant of the green fluorescent protein was used in a time‐lapse imaging study. Cell aggregation began on PHBHHx at 2 h, and became obvious at 4 h. Furthermore, cells on PHBHHx displayed higher metabolic activities measured by MTT assay than that on tissue culture plate. More importantly, insulin gene expression as well as extracellular secretion was upregulated after growth on PHBHHx for 72 h. Thus, PHBHHx can be a strong candidate for islet transplantation. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010