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Platelet leukocyte gel facilitates bone substitute growth and autologous bone growth in a goat model
Author(s) -
Everts Peter A. M.,
Delawi Diyar,
Mahoney Christine Brown,
van Erp Albert,
Overdevest Eddy P.,
van Zundert André,
Knape Johannes T. A.,
Dhert Wouter J. A.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32372
Subject(s) - biomedical engineering , calcium , bone growth , platelet , materials science , bone formation , medicine , metallurgy
The aim of this study is to evaluate multiple conditions on the formation of bone growth in a goat model. We prepared from a unit of whole blood, platelet‐leukocyte gel (PLG) to stimulate bone formation, based on the release of platelet growth factors. Two 3‐compartment cages containing autologous bone, calcium phosphate, and trabecular metal were implanted onto goat spinal transverse processes. One cage was treated with PLG, prepared according to a standardized protocol. An untreated cage served as a control. To monitor bone formation overtime, fluorochrome markers were administered at 2, 3, and 5 weeks. Animals were sacrificed at 9 weeks after implantation. Bone growth in these 3‐compartments cages was examined by histology and histomorphometry of nondecalcified sections using traditional light and epifluorescent microscopy. Compared to the control samples, bone growth in the PLG‐treated autologous bone and calcium phosphate samples was significantly more. Fairly little bone growth was seen in PLG treated or untreated trabecular metal scaffolds. The results obtained from this goat model suggest a potential role for the application of autologous PLG during surgeries in which autologous bone grafts or calcium phosphate scaffolds are used. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010