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In vitro cartilage formation using TGF‐β‐immobilized magnetic beads and mesenchymal stem cell‐magnetic bead complexes under magnetic field conditions
Author(s) -
Motoyama Mitsuru,
Deie Masataka,
Kanaya Atsushi,
Nishimori Makoto,
Miyamoto Ayato,
Yanada Shinobu,
Adachi Nobuo,
Ochi Mitsuo
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32365
Subject(s) - chondrogenesis , bead , mesenchymal stem cell , materials science , cartilage , biomedical engineering , anatomy , biology , composite material , microbiology and biotechnology , medicine
We evaluated the efficacy of transforming growth factor (TGF)‐β‐immobilized magnetic beads for chondrogenesis in vitro using a mesenchymal stem cell (MSC) delivery system and an external magnetic force (EMF). MSCs isolated from the bone marrow of Sprague Dawley rats were mixed with carboxyl group‐combined magnetic beads (Ferri Sphere 100C®) coated with anti‐rat CD44 mouse monoclonal antibodies. TGF‐β3 (10 and 1 ng/mL) was attached magnetically to such other Ferri Sphere 100C® beads via an amide bond formed between a primary amino group on the TGF‐β3 and the carboxyl groups on the surface of the beads. MSC‐magnetic bead complexes were centrifuged to form a pellet and cultured in chondrogenic differentiation medium (CDM) supplemented with either 10 or 1 ng/mL TGF‐β‐immobilized magnetic beads (10 or 1 ng/mL TGF‐β‐immobilized magnetic bead groups) or in CDM supplemented with 1 or 10 ng/mL TGF‐β (1 or 10 ng/mL TGF‐β group). TGF‐β‐immobilized magnetic beads were gathered effectively under an EMF. Chondrogenesis was achieved from the MSC‐magnetic bead complexes in the presence of 1 ng/mL TGF‐β‐immobilized magnetic beads. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010