Preparation and characterization of polyethylenimine‐coated Fe 3 O 4 ‐MCM‐48 nanocomposite particles as a novel agent for magnet‐assisted transfection | Zendy

Yiu Humphrey H. P. | Zendy; McBain Stuart C. | Zendy; Lethbridge Zoe A. D. | Zendy; Lees Martin R. | Zendy; Dobson Jon | Zendy

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Preparation and characterization of polyethylenimine‐coated Fe 3 O 4 ‐MCM‐48 nanocomposite particles as a novel agent for magnet‐assisted transfection

Author(s) -

Yiu Humphrey H. P.,

McBain Stuart C.,

Lethbridge Zoe A. D.,

Lees Martin R.,

Dobson Jon

Publication year - 2010

Publication title -

journal of biomedical materials research part a

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.849

H-Index - 150

eISSN - 1552-4965

pISSN - 1549-3296

DOI - 10.1002/jbm.a.32363

Subject(s) - materials science , polyethylenimine , superparamagnetism , nanocomposite , nanoparticle , magnetite , chemical engineering , thermal decomposition , magnetic nanoparticles , nanotechnology , particle size , mcm 41 , nuclear chemistry , transfection , magnetization , adsorption , organic chemistry , metallurgy , molecular sieve , chemistry , genetics , physics , quantum mechanics , magnetic field , engineering , biology , cell culture

A new type of magnetic nanoparticle was synthesized using mesoporous silica MCM‐48 as a template. Magnetite (Fe 3 O 4 ) nanocrystals were incorporated onto the MCM‐48 silica structure by thermal decomposition of iron(III) acetylacetonate. The particle size of these Fe 3 O 4 ‐MCM‐48 composite particles is around 300 nm with an iron oxide content of ca. 20% w/w. Measurements from SQUID magnetometry suggest that these nanoparticles possess superparamagnetic properties similar to those of Fe 3 O 4 nanoparticles. By coating positively charged polyethylenimine on to the surface, DNA can be bound onto the Fe 3 O 4 ‐MCM‐48 nanoparticles. Transfection studies showed that these PEI‐Fe 3 O 4 ‐MCM‐48 particles were highly effective as a transfection reagent, and a 400% increase of transfection efficiency compared with the commercial products was recorded. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010

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