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The effect of laminin peptide gradient in enzymatically cross‐linked collagen scaffolds on neurite growth
Author(s) -
Yao Li,
Damodaran Gopinath,
Nikolskaya Natalia,
Gorman Adrienne M.,
Windebank Anthony,
Pandit Abhay
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32359
Subject(s) - neurite , laminin , regeneration (biology) , microbiology and biotechnology , scaffold , cell adhesion , materials science , integrin , neural tissue engineering , adhesion , tissue engineering , biophysics , cell , biomedical engineering , chemistry , biology , extracellular matrix , biochemistry , in vitro , medicine , composite material
Abstract Guided neurite growth is critical in both peripheral nervous system and central nervous system nerve regeneration. Scaffolds that provide structural and guidance cues for neuronal cells have a potential role in neural regeneration application. Type I collagen is suitable to be processed as an engineered scaffold for nerve regeneration because of its biological and structural properties. A few previous studies have shown that cross‐linking of collagen scaffolds with microbial transglutaminase improves the mechanical strength and degradation properties of the scaffolds. It was shown that laminin 5 can regulate neurite outgrowth and extension. A motif (PPFLMLLKGSTR) in the human laminin 5 α3 chain is crucial for both integrin α3β1 receptor binding and cell adhesion. In the present work, we studied the guidance effect of a laminin peptide (PPFLMLLKGSTR) gradient in collagen and cross‐linked collagen scaffolds on neurite growth. Neurites of rat pheochromocytoma (PC12) cells showed a preferential growth toward the high laminin concentration level on the collagen scaffold, while the incorporation of laminin peptide in the scaffold did not influence neurite length of PC12 cells. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010