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Repair of segmental bone‐defect of goat's tibia using a dynamic perfusion culture tissue engineering bone
Author(s) -
Wang Chaofeng,
Wang Zhen,
Li Aimin,
Bai Feng,
Lu Jianxi,
Xu Shanglong,
Li Dichen
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32347
Subject(s) - materials science , biomedical engineering , tibia , perfusion , tissue engineering , process (computing) , anatomy , radiology , medicine , computer science , operating system
Segmental bone defect resulted from trauma or excision of bone tumor or pathology represents a common and significant clinical problem. In an attempt to solve this dilemma, we use β‐TCP combined with autologous bone marrow mesenchymal stem cells (auto‐BMSC) and cultured by dynamic perfusion to repair the segmental bone defects of goat's tibia. The β‐ TCP scaffolds combined with auto‐BMSC by dynamic perfusion bioreactor or in static state were respectively transplanted into the defect (30 mm) of the goat tibias. The X‐ray films were gathered and analyzed at the different time points. At 24 weeks post‐operation, tissue engineering bones implanted were analyzed by histology and Micro‐CT. Results show that the capacity of osteogenesis in experimental group was higher than that of control group by X‐ray, histological, and micro‐CT analysis ( p < 0.05). According to the study, we found that repair for segmental bone defects of tissue engineering bone cultured by dynamic perfusion culture bioreactor outweigh those cultured in static state. And we can conclude that this technology of tissue engineering bone will become a clinical method to the segmental bone‐defects repair in the future. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010