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Protein‐resistant polyurethane via surface‐initiated atom transfer radical polymerization of oligo(ethylene glycol) methacrylate
Author(s) -
Jin Zhilin,
Feng Wei,
Zhu Shiping,
Sheardown Heather,
Brash John L.
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32319
Subject(s) - atom transfer radical polymerization , materials science , protein adsorption , ethylene glycol , polymer chemistry , contact angle , methacrylate , adsorption , polyurethane , monomer , polymerization , polymer , x ray photoelectron spectroscopy , surface modification , chemical engineering , chemistry , organic chemistry , composite material , engineering
Protein‐resistant polyurethane (PU) surfaces were prepared by surface‐initiated simultaneous normal and reverse atom transfer radical polymerization (s‐ATRP) of poly(oligo(ethylene glycol) methacrylate) (poly (OEGMA)). Oxygen plasma treatment was employed for initial activation of the PU surface. The grafted polymer chain length was adjusted by varying the molar ratio of monomer to sacrificial initiator in solution from 5:1 to 200:1. The modified PU surfaces were characterized by water contact angle, X‐ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). Protein adsorption experiments from tris‐buffered saline (TBS) and plasma were carried out to evaluate the protein‐resistance of the surfaces. Adsorption from single and binary protein solutions as well as from plasma was significantly reduced after modification. Adsorption decreased with increasing poly(OEGMA) chain length. Fibrinogen (Fg) adsorption on the 200:1 monomer/initiator surface was in the range of 3–33 ng/cm 2 representing 96–99% reduction compared with the unmodified PU. Fg adsorption from 0.01–10% plasma was as low as 1–5 ng/cm 2 . Moreover, binary protein adsorption experiments using Fg and lysozyme (Lys) showed that protein size is a factor in the protein resistance of these surfaces. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2009