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In vitro elution of moxifloxacin from cancellous bone allografts
Author(s) -
Kanellakopoulou Kyriaki,
Sahinides Theophilos,
Tsaganos Thomas,
Galanakis Nearchos,
Giamarellou Helen,
GiamarellosBourboulis Evangelos J.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32316
Subject(s) - materials science , moxifloxacin , cancellous bone , biomedical engineering , elution , in vitro , composite material , chromatography , medicine , microbiology and biotechnology , surgery , biology , antibiotics , biochemistry , chemistry
The characteristics of cancellous bone allografts as carriers of moxifloxacin are described. Particles of cancellous bone were compressed into a wire‐mesh cylinder and impregnated into a solution of moxifloxacin for different time periods. Five replicas were impregnated for 1 h; another five for 24 h; and another five for 48 h. Impregnated allografts were then transferred into vials containing 5 ml of Mueller‐Hinton broth and incubated at 37°C. Broth was replaced daily. Concentrations of moxifloxacin in broth were determined after analysis by an high performance liquid chromatography system. Moxifloxacin was eluted at very high concentrations within the first days. Concentrations remained above 100 μg/ml until day 8 and above 40 μg/ml until day 20. It is concluded that cancellous bone allografts may allow the adequate in vitro elution of moxifloxacin. The latter results support their application in experimental models of osteomyelitis. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010