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Effects of mechanical stimuli on the synthesis of superficial zone protein in chondrocytes
Author(s) -
Kamiya T.,
Tanimoto K.,
Tanne Y.,
Lin Y. Y.,
Kunimatsu R.,
Yoshioka M.,
Tanaka N.,
Tanaka E.,
Tanne K.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32295
Subject(s) - messenger rna , elongation , blot , downregulation and upregulation , western blot , microbiology and biotechnology , cartilage , materials science , ultimate tensile strength , biophysics , chemistry , biology , anatomy , biochemistry , composite material , gene
Superficial zone protein (SZP) has been demonstrated to contribute to the boundary lubrication in synovial joints. This study was designed to clarify the modulation of SZP expression by mechanical stress in articular chondrocytes. Cyclic tensile strains of 7 and 21% cell elongation were applied to cultured chondrocytes obtained from porcine mandibular condylar cartilage. The mRNA levels of SZP, IL‐1β, and TGF‐β1 were examined by a quantitative real‐time PCR analysis. Protein level of SZP was examined by Western blotting. The SZP mRNA level was significantly upregulated after 12, 24, and 48 h by 7% elongation. Although SZP mRNA level was upregulated by 21% elongation after 12 h, it decreased to a lower level than the control after 48 h. The TGF‐β1 mRNA level exhibited an almost similar change to SZP. The IL‐1β mRNA level was not changed markedly by 7% elongation. However, the IL‐1β mRNA level was significantly increased by a 12‐h application of 21% elongation. Western blot analysis revealed that the SZP expression was increased by 7% elongation, but decreased remarkably by 21% elongation. It is suggested from these findings that the SZP expression level in the chondrocytes is enhanced by optimal mechanical stimuli, but inhibited by excessive loading partly affected by TGF‐β1 and IL‐1β, leading to the deterioration of joint lubrication. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010