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Injectable cell‐free template for bone‐tissue formation
Author(s) -
Bergman Kristoffer,
Engstrand Thomas,
Hilborn Jöns,
Ossipov Dmitri,
Piskounova Sonya,
Bowden Tim
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32289
Subject(s) - in vivo , hyaluronic acid , materials science , biomedical engineering , bone morphogenetic protein 2 , bone tissue , polyvinyl alcohol , histology , in vitro , viability assay , self healing hydrogels , bone morphogenetic protein , bone formation , tissue engineering , microbiology and biotechnology , anatomy , chemistry , pathology , biochemistry , biology , medicine , polymer chemistry , composite material , gene
Here we present a novel injectable hydrogel which forms a template for de novo formation of bone tissue. Hydrogel formation takes place in situ in less than 1 min by the cross‐linking of multifunctional hyaluronic acid and polyvinyl alcohol derivatives. Endogenous cells are recruited in vivo by incorporating bone morphogenetic protein‐2 (BMP‐2), a powerful promoter for osteogenic differentiation. The hydrogel was evaluated in vitro by performing a cell viability test and a release study and in vivo by a rat ectopic model. Examination by X‐ray, microcomputed tomography, and histology revealed a significant bone formation at the target site for gels containing BMP‐2, and a complete degradation was observed for gels without BMP‐2 four weeks after injection. There were no signs of inflammation or foreign body response in either group and we believe that this system has the potential as an off‐the‐shelf injectable to be used where bone tissue is needed. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009