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Improving the affinity of fibroblasts for bacterial cellulose using carbohydrate‐binding modules fused to RGD
Author(s) -
Andrade F. K.,
Moreira S. M G.,
Domingues L.,
Gama F. M. P.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32284
Subject(s) - bacterial cellulose , cellulose , cellulosome , extracellular matrix , adhesion , clostridium thermocellum , cell adhesion , materials science , biochemistry , carbohydrate binding module , chemistry , cellulase , composite material
The attachment of cells to biomedical materials can be improved by using adhesion sequences, such as Arg‐Gly‐Asp (RGD), found in several extracellular matrix proteins. In this work, bifunctional recombinant proteins, with a Cellulose‐Binding Module (CBM), from the cellulosome of Clostridium thermocellum and cell binding sequences—RGD, GRGDY—were cloned and expressed in E.coli . These RGD‐containing cellulose binding proteins were purified and used to coat bacterial cellulose fibres. Its effect on the cell adhesion/biocompatibility properties was tested using a mouse embryo fibroblasts culture. Bacterial cellulose (BC) secreted by Gluconacetobacter xylinus (= Acetobacter xylinum ) is a material with unique properties and promising biomedical applications. CBMs adsorbs specifically and tightly on cellulose. Thus, they are a useful tool to address the fused RGD sequence (or other bioactive peptides) to the cellulose surface, in a specific and simple way. Indeed, fibroblasts exhibit improved ability to interact with bacterial cellulose sheets coated with RGD‐CBM proteins, as compared with cellulose treated with the CBM, that is, without the adhesion peptide. The effect of the several fusion proteins produced was analyzed. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010