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Monocyte inflammatory and matrix remodeling response modulated by grafted ECM‐derived ligand concentration
Author(s) -
Chung Amy S.,
Waldeck Heather,
Schmidt David R.,
Kao Weiyuan John
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32259
Subject(s) - extracellular matrix , monocyte , materials science , matrix metalloproteinase , ligand (biochemistry) , adhesion , proinflammatory cytokine , cell adhesion , gelatin , peptide , peg ratio , biophysics , microbiology and biotechnology , biochemistry , chemistry , biology , inflammation , immunology , receptor , finance , economics , composite material
Ligands presented on biomaterials are a common method to facilitate and control the host response. In a gelatin and polyethylene glycol diacrylate (PEGdA) based semi‐interpenetrating network (sIPN), the effects of extracellular matrix (ECM)‐derived peptide amount on monocyte adhesion and subsequent protein and mRNA expression were examined. Peptide amount on the sIPN surface was controlled by varying the wt % ratio of the peptide‐PEG grafted gelatin to PEGdA. We hypothesized that increasing bioactive peptide amount would modulate human blood‐derived monocyte adhesion, cytokine expression, and gene regulation. Monocyte adhesion, release of gelatin degrading proteases matrix metalloprotease‐2 (MMP‐2), matrix metalloprotease‐9 (MMP‐9), and proinflammatory protein interleukin‐1β (IL‐1β), and mRNA expression of these proteins were evaluated. We found RGD‐PEG grafted sIPNs with higher surface RGD concentrations showed increased adherent density. MMP‐2 and IL‐1β protein release was also influenced by the ligand concentration, as initial increase in protein concentration was observed at higher ligand concentrations. MMP‐9 protein showed an initial increase that subsided then increased. A decreased IL‐1β protein and mRNA expression was observed over time but MMP‐2 mRNA was not detected at any time though MMP‐2 protein concentrations showed an initial burst. Hence, monocyte behavior was modulated by surface ligand identity in tandem with ligand concentration. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009