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PEGylation strategies for active targeting of PLA/PLGA nanoparticles
Author(s) -
Betancourt Tania,
Byrne James D.,
Sunaryo Nicole,
Crowder Spencer W.,
Kadapakkam Meena,
Patel Shefali,
Casciato Shelly,
BranPeppas Lisa
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32247
Subject(s) - materials science , pegylation , plga , nanoparticle , nanotechnology , chemical engineering , polyethylene glycol , engineering
This work evaluates various techniques for the incorporation of poly(ethylene glycol) (PEG) onto biodegradable nanoparticles (NPs) of poly(lactic‐ co ‐glycolic acid) (PLGA) or poly(lactic acid) (PLA) with the purpose of providing a functional site for surface conjugation of targeting agents and for improving surface properties. The techniques compared were based on NP preparation with blends of PLGA and poloxamer or with block copolymers of PLGA/PLA with PEG. Blending of PLGA with poloxamer 407 resulted in the incorporation of the latter to up to a 43 wt % content. Direct conjugation of heterofunctional NH 2 ‐PEG‐COOH to the surface of premade NPs was not highly effective. Preparation of copolymers of PLGA with PEG was determined to be more effective and versatile by polymerization of lactide and glycolide dimers onto the hydroxyl group of heterofunctional OH‐PEG‐COOH than by conjugation of the premade polymers with carbodiimide chemistry. NPs prepared with these copolymers confirmed the surface localization of PEG and proved to be useful for conjugation of mouse immumoglobulin as a model targeting agent. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009