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Culture of skeletal muscle cells in unprecedented proximity to a gold surface
Author(s) -
Coletti Dario,
Scaramuzzo Francesca A.,
Montemiglio Linda C.,
Pristerà Alessandro,
Teodori Laura,
Adamo Sergio,
Barteri Mario
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32243
Subject(s) - myogenesis , cysteamine , myogenin , c2c12 , myocyte , materials science , myosin , cell culture , biophysics , microbiology and biotechnology , skeletal muscle , tissue engineering , nanotechnology , biology , anatomy , biochemistry , genetics
Culturing of skeletal muscle cells on conductive surfaces is required to develop electronic device–muscle junctions for tissue engineering and medical applications. We characterized from a molecular and morphological point of view myogenic cells cultured on gold and on cysteamine‐coated gold, as compared to the standard plastic for cell culture. Our results show that cell proliferation and survival are comparable between cells grown on either of the gold surface or plastic. The majority of the cells cultured on gold surfaces retain the ability to respond to differentiation cues, as shown by nuclear translocation of myogenin. Following terminal differentiation, the myotubes cultured on cysteamine‐coated gold resemble myotube cultures obtained on plastic for the size and orientation of the myotube bundles retaining most of myosin expression; on the contrary, the myotube cultures on gold show a clumped morphology, likely due to repulsive cell‐substratum interaction resulting in aberrant differentiation. On the basis of the aforementioned evidences, the culture of muscle cells on cysteamine‐coated gold represents an advance with respect to previously reported substrata. The cysteamine self‐assembled monolayer coating is a simple approach to accomplish cultures of myotubes in unprecedented tight proximity to conductive surfaces. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009