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Responses in vivo to purified poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) implanted in a murine tibial defect model
Author(s) -
Wu CK. A.,
Pettit A. R.,
Toulson S.,
Grøndahl L.,
Mackie E. J.,
Cassady A. I.
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32238
Subject(s) - materials science , biocompatibility , biomaterial , in vivo , implant , biomedical engineering , bone tissue , surgery , biology , medicine , nanotechnology , microbiology and biotechnology , metallurgy
Effective bone biomaterials provide structural support for bone regeneration and elicit minimal inflammatory or toxic effects in vivo . Poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) (PHBV) is a bacterially derived biodegradable polymer that possesses suitable mechanical strength for use as a bone biomaterial and has a slow rate of degradation in biological environments. Our previous in vitro study showed that many PHBV preparations are contaminated with bacterial lipopolysaccharide, and we developed a purification procedure to substantially remove it. Here, we have evaluated the in vivo biocompatibility of PHBV purified by H 2 O 2 treatment and solvent extraction. We utilized a murine tibial defect model consisting of a hole drilled through the diameter of the tibial diaphysis into which nonporous cylindrical plugs of purified PHBV were implanted. The animals were sacrificed at 1 week and 4 weeks postsurgery, and tibiae were examined using histological staining. The PHBV implant induced a mild inflammatory response 1 week after injury, which persisted for 4 weeks. Granuloma type tissues formed only when the implant protruded into the overlaying tissue. Woven bone formation occurred adjacent to the implant, which gave rise to lamellar bone and stabilized the implant indicating that the PHBV did not affect this process. Our data validated the murine defect model and indicate that solid PHBV induces a mild tissue reaction with bone deposition adjacent to the implant with no fibrous tissue present at 4 weeks post surgery. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009