Premium
Influence of poly( D,L ‐lactic‐ co ‐glycolic acid) microsphere degradation on arteriolar remodeling in the mouse dorsal skinfold window chamber
Author(s) -
Nickerson Meghan M.,
Song Ji,
Shuptrine Casey W.,
Wieghaus Kristen A.,
Botchwey Edward A.,
Price Richard J.
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32209
Subject(s) - materials science , glycolic acid , microsphere , lactic acid , dorsum , biophysics , degradation (telecommunications) , window (computing) , biomedical engineering , chemical engineering , composite material , anatomy , biology , medicine , genetics , bacteria , engineering , telecommunications , computer science , operating system
Poly( D,L ‐lactic‐ co ‐glycolic acid) (PLGA) is a biodegradable polymer that is widely used for drug delivery. However, the degradation of PLGA alters the local microenvironment and may influence tissue structure and/or function. Here, we studied whether PLGA degradation affects the structure of the arteriolar microcirculation through arteriogenic expansion of maximum lumenal diameters and/or the formation of new smooth muscle‐coated vessels. Single microspheres comprised of 50:50 PLGA (521 ± 52.7 μm diameter), 50:50 PLGA with bovine serum albumin (BSA) (547 ± 62.2 μm), 85:15 PLGA (474 ± 52.6 μm), or 85:15 PLGA with BSA (469 ± 57.2 μm) were implanted into mouse dorsal skinfold window chambers, and longitudinal arteriolar diameter measurements were made in the presence of a vasodilator (10 −4 M adenosine) over 7 days. At the end of the 7‐day period, the length density of all smooth muscle‐coated microvessels was also determined. Implantation of the window chamber alone elicited a 22% increase in maximum arteriolar diameter. However, the addition of 85:15 and 50:50 PLGA microspheres, bearing either BSA or no protein, elicited a significant enhancement of this arteriogenic response, with final maximum arteriolar diameters ranging from 36 to 46% more than their original size. Interestingly, the influence of PLGA degradation on microvascular structure was limited to lumenal arteriolar expansion, as we observed no significant differences in length density of smooth muscle‐coated microvessels. We conclude that the degradation of PLGA microspheres may elicit an arteriogenic response in subcutanteous tissue in the dorsal skinfold window chamber; however, it has no apparent effect on the total length of smooth muscle‐coated microvasculature. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009