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Behavior of rat periodontal ligament cells on fibroblast growth factor‐2‐immobilized titanium surfaces treated by plasma modification
Author(s) -
Kokubu Eitoyo,
Yoshinari Masao,
Matsuzaka Kenichi,
Inoue Takashi
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32201
Subject(s) - periodontal fiber , titanium , alkaline phosphatase , materials science , fibroblast growth factor , quartz crystal microbalance , fibroblast , vascular endothelial growth factor , subculture (biology) , biophysics , biomedical engineering , chemistry , biochemistry , biology , medicine , dentistry , in vitro , vegf receptors , enzyme , microbiology and biotechnology , receptor , organic chemistry , adsorption , metallurgy
The aim of this study was to investigate the behavior of rat periodontal ligament (PDL) cells cultured on fibroblast growth factor‐2 (FGF‐2)‐immobilized titanium surfaces treated by oxygen (O 2 ) plasma. We used cell disks (15 mm in diameter), and 35‐mm culture dishes sputter‐coated with titanium. These were treated with oxygen plasma and dipped in FGF‐2 solution. Immobilized FGF‐2 was visualized with a confocal laser‐scanning microscope, and its weight was calculated to be ˜22.6 ng/cm 2 using a quartz crystal microbalance‐dissipation apparatus. The PDL cells were obtained from rat incisors. Cells from fourth subculture were seeded onto the FGF‐2‐immobilized titanium surface. Proliferation ratio, alkaline phosphatase (ALP) activity, and expressions of type I collagen and vascular endothelial growth factor (VEGF) mRNAs were evaluated. Proliferation ratio and expressions of type I collagen and VEGF mRNAs were significantly higher, whereas ALP activity was significantly lower in FGF‐2‐immobilized cells than in control group ( p < 0.05). These findings suggest that oxygen plasma modification can immobilize FGF‐2 onto a titanium surface. Immobilized FGF‐2, although inferior to culture medium with FGF‐2, influenced the proliferation of PDL cells and might have promoted collagen and vascular synthesis. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009