Premium
Cytocompatibility studies of an in situ photopolymerized thermoresponsive hydrogel nanoparticle system using human aortic smooth muscle cells
Author(s) -
Sabnis Abhimanyu,
Rahimi Maham,
Chapman Christopher,
Nguyen Kytai T.
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32194
Subject(s) - photoinitiator , photopolymer , materials science , self healing hydrogels , cytotoxicity , radical , ethylene glycol , nanoparticle , polymer chemistry , chemistry , polymer , polymerization , nanotechnology , organic chemistry , composite material , biochemistry , monomer , in vitro
We have been investigating thermoresponsive hydrogel nanoparticle composite networks to develop photopolymerized hydrogels to deliver drugs for prevention of restenosis after angioplasty. These composite systems can form a gel under physiological conditions and release drugs in response to temperature changes. Our novel system, consisting of poly( N‐ isopropylacrylamide) thermoresponsive nanoparticles, photo cross‐linker poly(ethylene glycol) diacrylate, and UV photoinitiator, 2‐hydroxy‐1‐[4‐(2‐hydroxyethoxy) phenyl]‐2‐methyl‐1‐propanone‐1‐one (Irgacure 2959), would be photopolymerized in situ in the presence of UV light. The focus of this study was to evaluate the effects of photoinitiator and UV exposure on human aortic smooth muscle cells (HASMCs). We found that the exposure to UV light did not significantly affect the cellular survival within doses required for photopolymerization. The photoinitiator was cytocompatible at low concentrations (≤0.015% w/v); however, cytotoxicity increased with increasing photoinitiator concentrations. In addition, free radicals formed in the presence of a photoinitiator and UV light caused significant levels of cell death. An antioxidant (free radical scavenger), ascorbic acid, added to the cell media, significantly improved relative cell survival but increased the hydrogel gelation time. Finally, HASMC survival when exposed to all potential cytotoxic components was also evaluated by exposing HASMCs to media incubated with our composite hydrogels. In summary, our studies show that the photoinitiator and free radicals are responsible for the cytotoxicity on HASMCs, and the addition of antioxidants can significantly reduce these harmful effects. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009