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Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering
Author(s) -
Diao Yinze,
Ma Qingjun,
Cui Fuzhai,
Zhong Yanfeng
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32186
Subject(s) - mesenchymal stem cell , cd90 , bone marrow , tissue engineering , microbiology and biotechnology , cd34 , stem cell , cord lining , materials science , stem cell transplantation for articular cartilage repair , biology , immunology , biomedical engineering , adult stem cell , in vitro , medicine , endothelial stem cell , biochemistry
Mesenchymal stem cells (MSCs) are ideal seed cells for bone tissue engineering. However, intrinsic deficiencies exist for the autologous transplantation strategy of constructing artificial bone with MSCs derived from bone marrow of patients. In this study, MSCs‐like cells were isolated from human umbilical cords and were expanded in vitro . Flow cytometric analysis revealed that cells from the fourth passage were positive for CD29, CD44, CD71, CD73, CD90, and CD105 whereas they were negative for CD14, CD34, CD45, and CD117. Furthermore, these cells expressed HLA‐A, B, C (MHC‐I), but not HLA‐DP, DQ, DR (MHC‐II), or costimulatory molecules such as CD80 and CD86. Following incubation in specific inductive media for 3 weeks, cultured cells were shown to possess potential to differentiate into adipogenic, osteogenic or chondrogenic lineages in vitro . The umbilical cord‐derived MSCs (UC‐MSCs) were loaded with a biomimetic artificial bone scaffold material before being implanted subcutaneously in the back of Balb/c nude mice for four to twelve weeks. Our results revealed that UC‐MSCs loaded with the scaffold displayed capacity of osteogenic differentiation leading to osteogenesis with human origin in vivo . As a readily available source of seed cells for bone tissue engineering, UC‐MSCs should have broad application prospects. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009