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Thin‐film assembly of diethanolamine‐based lipidic material as potential gene carrier in mouse embryonic neural stem cells
Author(s) -
Kusumoto Kenichi,
Yamashita Satoko,
Nagata Takahiro,
Ido Takeshi,
Hamachi Itaru,
Akao Tetsuyuki
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32159
Subject(s) - neural stem cell , diethanolamine , embryonic stem cell , stem cell , materials science , gene delivery , gene , cytotoxicity , microbiology and biotechnology , biology , transfection , biochemistry , in vitro
Understanding of lipidic materials used for gene delivery system is essential for the effective design and development of potential applications in basic and therapeutic research. This study aimed to evaluate the biological activity of totally synthesized ditetradecylacetyldiethanolaminetrimethylammonium (TMA‐C2‐DEA‐C14) as gene carriers for neural stem cells. The transfer abilities were estimated by expressing green fluorescent protein (GFP) in mouse embryonic neural stem cells. Here, we demonstrate that lipidic assembly of TMA‐C2‐DEA‐C14, which was self‐organized by incubation in water for a month at 25°C, can provide an efficient gene delivery with low cytotoxicity (∼40% of GFP‐expressed neural stem cells). However, when dispersed by ultrasonication, TMA‐C2‐DEA‐C14 showed low effect (∼4%). Moreover, electron microscopic analysis showed that TMA‐C2‐DEA‐C14 assembly is characterized by thin‐film structures with polygonal shapes (∼2.7 μm), and after association with DNA, their structures dramatically changes to form liposome complexes that can effectively deliver DNA into the cellular cytoplasm of neural stem cells. Thus, TMA‐C2‐DEA‐C14 assembly identified in this study was determined to have an effective activity as gene carriers for primary neural stem cells. Our findings suggest that this approach can serve as a novel model for the development of lipidic materials on nonviral gene delivery system. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009