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Pulsed electric field as a potential new method for microbial inactivation in scaffold materials for tissue engineering: The effect on collagen as a scaffold
Author(s) -
Smith Sharon,
Griffiths Sarah,
MacGregor Scott,
Beveridge Joe,
Anderson John,
van der Walle Chris,
Grant M. Helen
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32150
Subject(s) - scaffold , sterilization (economics) , materials science , tissue engineering , biomedical engineering , biophysics , biology , medicine , monetary economics , economics , foreign exchange market , foreign exchange
Hybrid scaffolds for tissue engineering are becoming increasingly complex through incorporation of biologically active biomacromolecules. There is a need to develop a compatible sterilization method that is capable of killing microorganisms, without adversely affecting the labile scaffold biomaterials or biomacromolecular components. Pulsed electric field (PEF) treatment has been successful as a nonthermal microbial inactivation–pasteurization method within the food industry. We have previously demonstrated that PEF treatment inactivates E. coli seeded in collagen gels. Here, we show that PEF treatment does not affect the structural integrity of the collagen molecule or its adsorption to polystyrene and hydroxyapatite surfaces. Moreover, osteoblast cells cultured on PEF‐treated collagen, which was coated onto two‐ and three‐dimensional scaffolds, retained their normal morphology, growth rate, and functionality. PEF treatment, therefore, shows great potential to be used as a sterilization method for collagen‐based biomaterials. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009