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Development of water‐insoluble chitosan patch scaffold to repair traumatic tympanic membrane perforations
Author(s) -
Kim Jang Ho,
Bae JoonHo,
Lim Ki Taek,
Choung PillHoon,
Park JungSub,
Choi Seong Jun,
Im Ae Lee,
Lee Eung Tae,
Choung YunHoon,
Chung Jong Hoon
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32119
Subject(s) - chitosan , materials science , tympanoplasty , biocompatible material , in vivo , biomedical engineering , scaffold , biomaterial , membrane , wound healing , tissue engineering , ultimate tensile strength , tympanic membrane perforation , composite material , surgery , chemistry , medicine , nanotechnology , biochemistry , microbiology and biotechnology , biology
Perforated tympanic membranes (TM) and otitis media can be managed with a paper patch or tympanoplasty. However, a paper patch is not biocompatible and tympanoplasty requires complex aseptic surgical procedures. A novel biocompatible patch with a water‐insoluble chitosan as the main component was prepared. Optimal mechanical characteristics of a water‐insoluble chitosan patch scaffold (CPS) was ∼40 μm in thickness, 7 MPa in tensile strength, and 107% in percent elongation, even though the characteristics varied significantly depending on the concentrations of chitosan and glycerol. SEM of the CPSs showed a very smooth surface as compared with that of the paper patches. These CPSs showed no cytotoxicity and had a stimulating effect on the proliferation of TM cells in in vitro study. In in vivo study, 4 (21.1%) and 17 (89.5%) TMs out of 19 adult rats with CPSs showed no perforations at 1 and 2 weeks, respectively. However, left control TMs showed healing of 0 (0%) at 1 week and 18 (94.7%) at 2 weeks. TEM findings of regenerated eardrums using CPSs showed thinner, smoother, and more compact tissues than spontaneously healed eardrums. A CPS was more effective than spontaneous healing to repair traumatic TM perforations. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009