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Synthesis and evaluation of collagen–chitosan–hydroxyapatite nanocomposites for bone grafting
Author(s) -
Wang Xiaoliang,
Wang Xiaomin,
Tan Yanfei,
Zhang Bo,
Gu Zhongwei,
Li Xudong
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32087
Subject(s) - nanocomposite , materials science , fourier transform infrared spectroscopy , chitosan , grafting , in situ , chemical engineering , microstructure , osteoblast , nuclear chemistry , composite material , polymer , organic chemistry , chemistry , in vitro , biochemistry , engineering
Abstract Incorporation of hydroxyapatite (HA) into the matrix of collagen (Col) and chitosan (Chi) by in situ synthesis was introduced to prepare nanocomposites. Structural investigations of the pure Col–Chi mixture validated the influence of Chi on Col assembly, but the molecular interactions between Col and Chi was partially depressed during the intervention of in situ HA synthesis, as revealed by FTIR and DSC analyses. A series of Col–Chi–HA (CCHA) nanocomposites with varying HA content were thereby prepared by a sequential method, involving in situ synthesis in the Col–Chi system, then gelling at 25°C and subsequently washing the resultant elastic gel followed by dehydration consolidation. The structural characteristics and biological properties of the dehydrated CCHA nanocomposites were further evaluated by using XRD, FTIR, TG, and SEM analyses and the osteoblast culture experiment. Formation of a well integrated microstructure of organic fibers (ca. 90 nm in size) and dense matrix including inorganic aggregates (less than 30 nm in size) was found in these nanocomposites. Rat Ros 17/2.8 Osteoblasts proliferated and attached well on the surface of both CCHA nanocomposite and Col–Chi mixture. These results indicated that in situ HA synthesis in the Col–Chi system provided a feasible route for bone grafting nanocomposites. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009