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Tetraglyme coatings reduce fibrinogen and von Willebrand factor adsorption and platelet adhesion under both static and flow conditions
Author(s) -
Zhang Min,
Horbett Thomas A.
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32085
Subject(s) - adhesion , adsorption , materials science , platelet , fibrinogen , protein adsorption , platelet adhesiveness , platelet activation , von willebrand factor , biophysics , analytical chemistry (journal) , chemical engineering , composite material , chromatography , chemistry , biochemistry , organic chemistry , immunology , polymer , platelet aggregation , engineering , biology
Previous studies have showed that radio‐frequency plasma deposited tetraglyme coatings greatly reduced fibrinogen adsorption (Γ Fg ) from highly diluted plasmas (0.1 and 1%) and subsequent platelet adhesion under static conditions. In this study, the protein resistant properties of tetraglyme were re‐examined with high‐concentration plasma, and subsequent platelet adhesion was measured under both static and flow conditions. The resistance of tetraglyme to vWf adsorption (Γ vWf ) and the role of vWf in platelet adhesion under flow were also investigated. Γ Fg and Γ vWf were measured with 125 I radiolabeled proteins. Flow studies were done at shear rates of 50 or 500 s −1 by passing a platelet/red cell suspension through a GlycoTech flow chamber. When adsorbed from a series of increasing plasma concentrations, the adsorption of both proteins to tetraglyme increased steadily, and did not show a peak at intermediate dilutions, i.e., there was no Vroman effect. When plasma concentration was less than 10%, the tetraglyme surface was highly nonfouling, exhibiting ultralow Γ Fg (less than 5 ng/cm 2 ) and extremely low platelet adhesion under both static and flow conditions. However, when the adsorption was done from 100% plasma, Γ Fg was much higher (∼85 ng/cm 2 ), indicating that tetraglyme surface may not be sufficiently protein‐resistant in the physiological environment. To correlate platelet adhesion under flow with Γ Fg and Γ vWf , a series of tetraglyme surfaces varying in ether content and protein adsorption was created by varying deposition power. On these surfaces, platelet adhesion at low shear rate depended only on the amount of Γ Fg , but under high shear, both Γ Fg and Γ vWf affected platelet adhesion. In particular, it was found that Γ vWf must be reduced to less than 0.4 ng/cm 2 to achieve ultra low platelet adhesion under high shear. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009