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Periodontal ligament cellular structures engineered with electrospun poly( DL ‐lactide‐ co ‐glycolide) nanofibrous membrane scaffolds
Author(s) -
Inanç Bülend,
Arslan Y. Emre,
Seker Sükran,
Elçin A. Eser,
Elçin Y. Murat
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32066
Subject(s) - periodontal fiber , materials science , plga , bone sialoprotein , tissue engineering , nanofiber , biomedical engineering , electrospinning , viability assay , cementum , cell adhesion , adhesion , biomaterial , dental alveolus , fibroin , osteocalcin , cell , nanotechnology , dentistry , chemistry , silk , alkaline phosphatase , composite material , dentin , medicine , nanoparticle , enzyme , polymer , biochemistry
Periodontal tissue engineering is expected to overcome the limitations associated with the existing regenerative techniques for the treatment of periodontal defects involving alveolar bone, cementum, and periodontal ligament. Cell‐based tissue engineering approaches involve the utilization of in vitro expanded cells with regenerative capacity and their delivery to the appropriate sites via biomaterial scaffolds. The aim of this study was to establish living periodontal ligament cell‐containing structures on electrospun poly( DL ‐lactic‐ co ‐glycolic acid) (PLGA) nanofiber membrane scaffolds, assess their viability and characteristics, and engineer multilayered structures amenable to easy handling. Human periodontal ligament (hPDL) cells were expanded in explant culture and then characterized morphologically and immunohistochemically. PLGA nanofiber membranes were prepared by the electrospinning process; mechanical tensile properties were determined, surface topography, nanofiber size, and porosity status were investigated with SEM. Cells were seeded on the membranes at ∼50,000 cell/cm 2 and cultured for 21 days either in expansion or in osteogenic induction medium. Cell adhesion and viability were demonstrated using SEM and MTT, respectively, and osteogenic differentiation was determined with IHC and immunohistomorphometric evaluation of osteopontin, osteocalcin, and bone sialoprotein marker expression. At days 3, 6, 9, and 12 additional cell/membrane layers were deposited on the existing ones and multilayered hybrid structures were established. Results indicate the feasibility of periodontal ligament cell‐containing tissue‐like structures engineering with PDL cells and electrospun nanofiber PLGA scaffolds supporting cell adhesion, viability and osteogenic differentiation properties of cells in hybrid structures amenable to macroscopic handling. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009