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Mineral apposition rates provide significant information on long‐term effects in BMP‐induced bone regeneration
Author(s) -
Schopper Christian,
Moser Doris,
SpassovaTzekova Else,
Russmueller Guenter,
Goriwoda Walter,
Lagogiannis Georgios,
Ewers Rolf,
Redl Heinz
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32012
Subject(s) - biomaterial , apposition , biomedical engineering , materials science , bone formation , bone morphogenetic protein 2 , dentistry , medicine , anatomy , chemistry , in vitro , biochemistry
In this study, a CaP biomaterial was used as a carrier for rhBMP‐2. Biomaterials were investigated in calvarial and femoral defects using a rabbit animal model, with unloaded biomaterials serving as control. Fluorochrome labels were administered at days 14 and 70. Specimens were retrieved after 12 weeks for histological analysis. When area fractions were assessed by conventional histomorphometry, no significant effect of rhBMP‐2 on the amounts of regenerated bone and residual biomaterial were seen by 12 weeks. After mineral appositional rate (MAR) measurement using double labels, calculation yielded significantly higher MARs for defects at both implantation sites, when compared with surrounding bone, whether or not biomaterials were loaded with rhBMP‐2. Analyzing the effect of rhBMP‐2, both defect sites showed significantly higher MARs in the rhBMP‐2 group. MARs of bone surrounding the defects had also been elevated significantly by rhBMP‐2 at calvarial and femoral implantation sites. It is concluded that MAR measurement is suitable to identify long‐term effects of rhBMP‐2 on bone formation at a time when conventional histomorphometry using fractional area determination is inadequate. Also, by MAR assessment, effects of rhBMP‐2 on surrounding bone can be documented. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009