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Controlled release of bisphosphonate from a calcium phosphate biomaterial inhibits osteoclastic resorption in vitro
Author(s) -
Faucheux C.,
Verron E.,
Soueidan A.,
Josse S.,
Arshad M. D.,
Janvier P.,
Pilet P.,
Bouler J. M.,
Bujoli B.,
Guicheux J.
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31989
Subject(s) - bisphosphonate , materials science , bone resorption , calcium , in vitro , resorption , in vivo , biophysics , biochemistry , chemistry , osteoporosis , medicine , biology , microbiology and biotechnology , metallurgy
Calcium phosphate biomaterials such as calcium deficient apatite (CDA) have been contemplated as carrier for delivery of bisphosphonate in bone tissues. In the present work, we have investigated the in vitro biological properties of Zoledronate‐loaded CDA. CDA was loaded with zoledronate according to a previously described coating process. 31 P MAS NMR spectra demonstrated the effective loading of zoledronate onto CDA. Using 14 C labeled zoledronate, we then demonstrated the in vitro release of zoledronate from CDA. In a first set of experiments, we confirmed that Zoledronate reduced the number of TRAP‐, vitronectin receptor‐, and F‐actin ring‐positive cells as well as the resorption activity of osteoclasts obtained from a total rabbit bone cell culture. Interestingly, Zoledronate‐loaded CDA and its extractive solutions decreased the osteoclastic resorption. Finally, zoledronate‐loaded CDA did not affect the viability and alkaline phosphatase activity of primary osteoblastic cells. These data demonstrate that CDA is effective for loading and release of zoledronate. The released zoledronate inhibited osteoclastic resorption without affecting osteoblasts. Our findings therefore suggest that such a drug delivery system would allow an increase in the efficiency of bisphosphonates by being locally available. Further experiments are now required to evaluate the in vivo antiresorptive activity of this concept. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009