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Cationic supramolecules consisting of oligoethylenimine‐grafted α‐cyclodextrins threaded on poly(ethylene oxide) for gene delivery
Author(s) -
Yang Chuan,
Li Hongzhe,
Wang Xin,
Li Jun
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31976
Subject(s) - cationic polymerization , polyethylenimine , materials science , gene delivery , ethylene oxide , transfection , polymer chemistry , cyclodextrin , drug delivery , cytotoxicity , biophysics , nanotechnology , polymer , organic chemistry , chemistry , biochemistry , in vitro , copolymer , gene , biology , composite material
In this study, three cationic polyrotaxanes composed of multiple oligoethylenimine‐grafted α‐cyclodextrin rings threaded on a poly(ethylene oxide) chain have been synthesized and characterized, and investigated for gene delivery. All three cationic polyrotaxanes could efficiently compact pDNA into small nanoparticles, with diameters ranging from 100 to 200 nm. In both BHK‐21 and MES‐SA cell lines, the transfection efficiency mediated by the cationic polyrotaxanes were comparable or even higher than that of branched polyethylenimine (PEI) with a molecular weight of 25 kDa, which is one of the most efficient gene‐delivery vectors to date. Moreover, the cationic polyrotaxanes showed much lower cytotoxicity than branched PEI (25 kDa). Hence, these cationic poly rotaxanes have high potentials as new carriers for gene delivery. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009