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Effect of dual growth factor delivery on chondrogenic differentiation of rabbit marrow mesenchymal stem cells encapsulated in injectable hydrogel composites
Author(s) -
Park Hansoo,
Temenoff Johnna S.,
Tabata Yasuhiko,
Caplan Arnold I.,
Raphael Robert M.,
Jansen John A.,
Mikos Antonios G.
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31948
Subject(s) - aggrecan , mesenchymal stem cell , self healing hydrogels , materials science , chondrogenesis , gelatin , growth factor , ethylene glycol , biomedical engineering , type ii collagen , transforming growth factor , microbiology and biotechnology , biophysics , chemistry , cartilage , biology , biochemistry , anatomy , pathology , polymer chemistry , osteoarthritis , medicine , articular cartilage , alternative medicine , receptor , organic chemistry
An injectable hydrogel composite consisting of oligo(poly(ethylene glycol)fumarate) (OPF) and gelatin microparticles has been developed as a novel carrier system for cells and growth factors. Rabbit marrow mesenchymal stem cells (MSCs) and gelatin microparticles (MPs) loaded with insulin‐like growth factor‐1 (IGF‐1), transforming growth factor‐β1 (TGF‐β1), or a combination of both growth factors were mixed with OPF, a poly(ethylene glycol)‐diacrylate crosslinker and the radical initiators ammonium persulfate and N , N , N ′, N ′‐tetramethylethylenediamine, and then crosslinked at 37°C for 8 min to form hydrogel composites. Hydrogel composites encapsulating rabbit marrow MSCs and blank MPs served as controls. At day 14, confocal fluorescent images of OPF hydrogels showed a strong aggregation of rabbit marrow MSCs when encapsulated with IGF‐1‐loaded MPs with or without TGF‐β1‐loaded MPs. Quantitative RT‐PCR results showed that rabbit marrow MSCs encapsulated with MPs loaded with TGF‐β1 or both TGF‐β1 and IGF‐1 had a significant increase in the expression of chondrocyte‐specific genes such as collagen type II and aggrecan at day 14 as compared with the control group. Specifically, samples with both TGF‐β1‐loaded MPs and IGF‐1‐loaded MPs exhibited a 121 ± 20‐fold increase of type II collagen gene expression and a 71 ± 24‐fold increase of aggrecan gene expression after 14 days of in vitro culture as compared with controls at day 0. These results suggest that hydrogel composites based on OPF and gelatin microparticles have great potential as carriers for MSCs and multiple growth factors for cartilage tissue engineering applications. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009