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Photoreversibility and photostability in films of octopus rhodopsin isolated from octopus photoreceptor membranes
Author(s) -
Paternolli Cristina,
Neebe Martin,
Stura Enrico,
Barbieri Francesca,
Ghisellini Paola,
Hampp Norbert,
Nicolini Claudio
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31925
Subject(s) - rhodopsin , biomaterial , materials science , membrane , octopus (software) , polyvinyl alcohol , biophysics , biology , optics , composite material , nanotechnology , biochemistry , chemistry , retinal , physics , computational chemistry
In this work, a new biomaterial resulting from the isolation of octopus rhodopsin (OR) starting from octopus photoreceptor membranes is presented. Mass spectroscopic characterization was employed in order to verify the presence of rhodopsin in the extract. Photoreversibility and photochromic properties were investigated using spectrophotometric measurements and pulsed light. Thin films of OR were realized using the gel‐matrix entrapment method in polyvinyl alcohol solution. The results indicate that the photoreversibility and the photostability of the OR in gel‐matrices are maintained. Several measurements were performed to test the stability of the resulting biomaterial in time and at room temperature. Preliminary tests demonstrate that the photoreversibility and the photostability are still found after few days from the biomaterial preparation and after the exposure for several hours at room temperature. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009