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A DNA transporter based on mesoporous silica nanospheres mediated with polycation poly(allylamine hydrochloride) coating on mesopore surface
Author(s) -
Qin Fei,
Zhou Yuchuan,
Shi Jianlin,
Zhang Yonglian
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31923
Subject(s) - allylamine , materials science , mesoporous silica , chemical engineering , mesoporous material , coating , surface modification , scanning electron microscope , polyelectrolyte , nanotechnology , polymer , organic chemistry , composite material , chemistry , catalysis , engineering
Mesoporous silica nanospheres (MPNSs) were synthesized by the sol–gel method and were investigated by X‐ray diffraction, nitrogen sorption, and transmission electron microscopy. The analyses showed that the MPNSs were highly dispersed, with a diameter range of 50–250 nm. Further, the polycation poly(allylamine hydrochloride) was coated on silica MPNSs to form a positively charged surface and to facilitate the loading of DNA molecules. The composite was successfully used as a intercellular carrier of DNA molecules, and this was confirmed by agarose gel electrophoresis and confocal laser scanning microscopy. Evidently, this composite has potential to be used as a carrier for a DNA delivery system. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009