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Assembly of multilayer PSS/PAH membrane on coherent alginate/PLO microcapsule for long‐term graft transplantation
Author(s) -
Leung Andy,
Trau Matt,
Nielsen Lars Keld
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31891
Subject(s) - materials science , term (time) , transplantation , membrane , biomedical engineering , medicine , surgery , biology , biochemistry , physics , quantum mechanics
Conventional alginate/poly‐ L ‐ornithine (AP) membranes used to immunoisolate foreign tissue transplants fail in long‐term transplantations of immortal cell lines. We have developed a novel layer‐by‐layer (LbL) membrane using polystyrene sulfonate and polyallylamine hydrochloride (PSS/PAH) on top of the coherent AP membrane. Assembly of the LbL membrane was followed by electrophoresis, and the surface morphologies and structure were characterized and examined by cryo‐scanning electron microscope and transmission electron microscopy. Unlike the standard AP membrane, the LbL membrane withstood the internal pressure generated by continuous cell proliferation of microencapsulated HEK‐293 and Min‐6 cells. The new membrane did not affect insulin secretion or diffusion by Min‐6 cells. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009