Premium
Platelet and endothelial adhesion on fluorosurfactant polymers designed for vascular graft modification
Author(s) -
Tang Chad,
Kligman Faina,
Larsen Coby C.,
KottkeMarchant Kandice,
Marchant Roger E.
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31888
Subject(s) - adhesion , platelet , materials science , cell adhesion , surface modification , integrin , peptide , platelet activation , biophysics , platelet adhesiveness , rgd motif , moiety , biochemistry , chemistry , receptor , stereochemistry , immunology , platelet aggregation , medicine , biology , composite material
A prominent failure mechanism of small diameter expanded polytetrafluoroethylene (ePTFE) vascular grafts is platelet‐mediated thrombosis. We have designed a surface modification for ePTFE consisting of a self‐assembling fluorosurfactant polymer (FSP) bearing biologically active ligands, including adhesive peptides and polysaccharide moieties. The goal of this biomimetic construct is to improve graft hemocompatibility by promoting rapid surface endothelialization, whereas minimizing platelet adhesion. Here we present a direct comparison of platelet and endothelial cell (EC) adhesion to FSPs containing one of three cell‐adhesion peptides: cyclic Arg‐Gly‐Asp‐D‐Phe‐Glu (cRGD), cyclic *Cys‐Arg‐Arg‐Glu‐Thr‐Ala‐Trp‐Ala‐Cys* (cRRE, *denotes disulfide bond cyclization), linear Gly‐Arg‐Gly‐Asp‐Ser‐Pro‐Ala (RGD), or a polysaccharide moiety: oligomaltose (M‐7), later designed to prevent nonspecific protein adhesion. Measurements of soluble peptide‐integrin binding indicated that cRRE exhibits very low affinity for the α IIb β 3 platelet fibrinogen receptor. Static and dynamic adhesion of washed, activated platelets on FSP‐modified surfaces revealed that M‐7 and cRRE promote significantly less platelet adhesion compared to RGD and cRGD FSPs, whereas EC adhesion was similar on all peptide FSPs and minimal on M‐7 FSP. These results illustrate the potential for ligands presented in a FSP surface modification to selectively adhere ECs with limited platelet attachment. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009