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Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries
Author(s) -
Schutte Robert J.,
ParisiAmon Andreina,
Reichert W. Monty
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31863
Subject(s) - chemokine , cytokine , materials science , monocyte , proinflammatory cytokine , polyethylene glycol , ccl3 , ccl2 , immunology , chemistry , inflammation , medicine , biochemistry
Cytokines, chemokines, and growth factors were assayed from the supernatants of monocytes and macrophages cultured on common biomaterials with a range of surface chemistries. TNF‐α, MCP‐1, MIP‐1α, IL‐8, IL‐6, IL‐1β, VEGF, IL‐1ra, and IL‐10 were measured from monocyte/macrophage cultures at different stages of activation and differentiation seeded onto polyethylene, polyurethane, expanded polytetrafluoroethylene, polymethyl methacrylate, and a hydrogel copolymer of 2‐hydroxyethyl methacrylate, 1‐vinyl‐2‐pyrrolidinone, and polyethylene glycol acrylate in tissue culture polystyrene (TCPS) plates. Empty TCPS wells and organo‐tin polyvinyl chloride served as “blanks” and positive controls, respectively. Results showed an overall increase in cytokine, chemokine, and growth factor production as monocytes are activated or differentiated into macrophages and that proinflammatory and anti‐wound healing cytokines and chemokines dominate this profile. However, cytokine production was only modestly affected by the surface chemistry of these four stable and noncytotoxic biomaterials. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009