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A novel method to prepare water‐dispersible magnetic nanoparticles and their biomedical applications: Magnetic capture probe and specific cellular uptake
Author(s) -
Yu Changjiang,
Zhao Jianjun,
Guo Yingzhi,
Lu Cailing,
Ma Xu,
Gu Zhongwei
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31786
Subject(s) - internalization , dispersity , materials science , magnetic nanoparticles , nanoparticle , oligonucleotide , superparamagnetism , nanotechnology , chemical engineering , biophysics , cell , biochemistry , chemistry , magnetic field , dna , polymer chemistry , magnetization , biology , physics , quantum mechanics , engineering
A novel and simple method to form water‐dispersed magnetic nanoparticles was successfully developed through glucosaminic acid‐surface modification of iron oxide nanoparticles. The resultant glucosaminic acid‐modified magnetic nanoparticles (GA‐MNPs) had not only good uniformity in spherical shape with diameter of about 10–13 nm, but also possessed excellent water‐dispersity and stability. In cell culture experiments, the internalization of GA‐MNPs into different kinds of cells was observed over a 5‐day period. The results indicated that the internalization of GA‐MNPs into mouse macrophage cells and mouse embryonic fibroblast cells was not observed after 40 h of culturing. However, the GA‐MNPs were internalized quickly into cancer cells after just 24 h of culturing. TEM images of the GA‐MNPs uptake in ECA‐109 cells were used to study the internalization mechanisms of GA‐MNPs and their distribution in ECA‐109 cells. Additionally, a water‐dispersed magnetic capture probe was prepared by immobilization of oligonucleotides onto GA‐MNPs, and the probe was used for detection and separation of their complementary oligonucleotides sequence. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 87A: 364–372, 2008