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Engineering tissue tubes using novel multilayered scaffolds in the rat peritoneal cavity
Author(s) -
Cao Yang,
Zhang Bing,
Croll Tristan,
Rolfe Barbara E.,
Campbell Julie H.,
Campbell Gordon R.,
Martin Darren,
CooperWhite Justin J.
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31781
Subject(s) - materials science , peritoneal cavity , elastin , biomedical engineering , tissue engineering , extracellular matrix , layer (electronics) , matrigel , chitosan , biomolecule , nanotechnology , cell , chemical engineering , anatomy , chemistry , microbiology and biotechnology , pathology , biology , medicine , biochemistry , engineering
Our aim was to develop novel scaffolds to engineer tissue tubes of smooth muscle‐like cells for autologous grafting. Small diameter tubular poly(lactic acid) scaffolds with randomly distributed, interconnected pores up to 100 μm were produced using a thermally induced phase separation method. The scaffolds were surface modified using various biomolecules via a layer‐by‐layer deposition technique, and implanted in the peritoneal cavities of rats. Histological analysis of scaffolds 3 weeks after implantation showed fully‐developed tissue capsules on their outer surfaces, with macrophage‐like cells present throughout the internal spaces. Surfaces coated in Matrigel supported the strongest cellular response whereas multilayer coatings with elastin, collagen I, collagen III, or chitosan outermost showed the lowest levels of cellular interaction. Although differences in capsule thickness and the presence or absence of cellularized layers on the inside and outside surfaces of the scaffolds were observed, none of these biomolecule coatings was able to overcome the foreign body response within the peritoneal cavity, even in the presence of a nonadsorptive HA undercoat. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2008