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Evaluation of bone healing with eggshell‐derived bone graft substitutes in rat calvaria: A pilot study
Author(s) -
Park JinWoo,
Bae SangRyul,
Suh JoYoung,
Lee DongHee,
Kim SangHyun,
Kim Hyungjun,
Lee ChongSoo
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31768
Subject(s) - calvaria , materials science , viability assay , calcium , bone healing , biomedical engineering , mtt assay , eggshell , osteoblast , dentistry , cell , anatomy , chemistry , medicine , in vitro , metallurgy , biochemistry , biology , ecology
The purpose of this study was to investigate the potential effectiveness of a surface‐modified natural calcium carbonate, hen eggshell (ES) as a bone graft substitute. The surface characteristics, cell viability on, and osteoconductivity of, particulated ES with and without hydrothermal treatment in phosphate solutions were evaluated. Hydrothermal treatment partially converted ES to calcium‐deficient hydroxyapatite (HA) with surface microstructure. MTT assay indicated higher osteoblast viability on surface‐modified ES compared with a commercially available bone substitute, anorganic bovine bone (Bio‐Oss, BO) ( p < 0.001). Histological and histomorphometric analysis showed significantly greater new bone formation and mineralized bone‐to‐graft contact of surface‐modified ES, especially with hydrothermally treated ES, compared with BO in 5‐mm diameter calvarial defects in rats at 4 and 8 weeks of healing ( p < 0.01). Complete bony bridging was more frequently found with hydrothermally treated ES. The results of this pilot study indicate the potential efficacy of surface‐modified particulated hen eggshell as an osteoconductive bone substitute in a rat calvarial defect model. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008