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Immobilized cytokines as biomaterials for manufacturing immune cell based vaccines
Author(s) -
Leclerc Claude,
Brose Claudia,
Nouzé Clémence,
Leonard Fransisca,
Majlessi Laleh,
Becker Sybille,
von Briesen Hagen,
LoMan Richard
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31751
Subject(s) - immune system , granulocyte macrophage colony stimulating factor , colony stimulating factor , materials science , macrophage , microbiology and biotechnology , context (archaeology) , cell , dendritic cell , cell culture , growth factor , immunology , cytokine , cancer research , biology , receptor , in vitro , stem cell , biochemistry , haematopoiesis , paleontology , genetics
Manufacturing of bioactive cell culture substrates represents a major challenge for the development of cell therapy for tissue repair and immune treatment of cancers, infectious diseases, or immunodeficiencies. In this context, we evaluated the capacity of several differentiation factors, including Granulocyte Macrophage Colony Stimulating Factor (GM‐CSF) and Macrophage Colony Stimulating Factor (M‐CSF), to drive differentiation of primary cell cultures, once immobilized on surfaces. We show that covalently immobilized signal factors fully retain their biological properties and efficiently promote differentiation of mouse and/or human precursor cells leading to the production of dendritic cells and macrophages. For GM‐CSF, we also show that the efficiency of receptor signaling is comparable using either soluble or tethered molecules. Such artificial bioactive interfaces are suitable for the development and automated production of cell‐based vaccines and therapies. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008