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Nanocrystalline diamond: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures
Author(s) -
Amaral M.,
Dias A. G.,
Gomes P. S.,
Lopes M. A.,
Silva R. F.,
Santos J. D.,
Fernandes M. H.
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31742
Subject(s) - materials science , biocompatibility , biomedical engineering , osteoblast , raman spectroscopy , confocal microscopy , nanocrystalline material , nanotechnology , bone tissue , mtt assay , in vitro , microbiology and biotechnology , metallurgy , medicine , biochemistry , chemistry , biology , physics , optics
Abstract Nanocrystalline diamond (NCD) has a great potential for prosthetic implants coating. Nevertheless, its biocompatibility still has to be better understood. To do so, we employed several materials characterization techniques (SEM, AFM, micro‐Raman spectroscopy) and cell culture assays using MG63 osteoblast‐like and human bone marrow cells. Biochemical routines (MTT assays, Lowry's method, ALP activity) supported by SEM and confocal microscopy characterization were carried out. We used silicon nitride (Si 3 N 4 ) substrates for NCD coatings based on a previous demonstration of the superior adhesion and tribological performance of these NCD coated ceramics. Results demonstrate an improved human osteoblast proliferation and the stimulation of differentiated markers, like ALP activity and matrix mineralization, compared with standard polystyrene tissue culture plates. The nanometric featuring of NCD, associated to its chemical affinity are key points for bone regeneration purposes. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008