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Therapeutic doses of radiation alter proliferation and attachment of osteoblasts to implant surfaces
Author(s) -
Ahmad Mansur,
Sampair Christopher,
NazmulHossain Abu N. M.,
Khuraeerja,
Nerness Andrew,
Wutticharoenmongkol Patcharaporn
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31737
Subject(s) - osseointegration , materials science , cell growth , ionizing radiation , osteoblast , irradiation , alkaline phosphatase , implant , andrology , chemistry , medicine , in vitro , biochemistry , surgery , enzyme , physics , nuclear physics
Osseointegration of implants in irradiated bone is inadequate. The effect of radiation on cell‐implant material interaction has not been adequately studied. The goal of this study was to investigate the effects of ionizing radiationon the proliferation, differentiation, and attachment of osteoblasts to commercially pure titanium (cpTi). Human fetal osteoblasts (hFOB) were irradiated either before or after plating in tissue culture (TC) dishes with or without cpTi disks. Radiation was single dose of 10 cGy, 25 cGy, 50 cGy, 1 Gy, 2 Gy, 4 Gy or 8 Gy. Cell proliferation was determined by counting trypsinized cells on 7 days after irradiation. Attachment of irradiated hFOB was measured indirectly by counting cells 2 and 6 h after plating. Differentiation was evaluated by alkaline phosphatase activity. Compared with nonirradiated sham controls, higher doses of radiation significantly reduced cell attachment and proliferation. Both proliferation and attachment were significantly lower on cpTi compared with TC. Attachment decreased based on the length of postirradiation period. Although differentiation was significantly enhanced by a dose of 8 Gy, proliferation was lowest. These initial studies show that effects of therapeutic doses of radiation on osteoblasts varied depending on the surface, time‐elapsed, and amount of radiation. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008