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Magnetic nanoparticles for improving cell invasion in tissue engineering
Author(s) -
Sasaki Takuro,
Iwasaki Norimasa,
Kohno Kenji,
Kishimoto Mikio,
Majima Tokifumi,
Nishimura ShinIchiro,
Minami Akio
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31724
Subject(s) - materials science , scaffold , tissue engineering , biocompatibility , magnetic nanoparticles , nanotechnology , chitosan , biomedical engineering , cell adhesion , cell , adhesion , nanoparticle , chemistry , medicine , composite material , biochemistry , metallurgy
It has been widely recognized that cells are seeded onto only the superficial layer of three‐dimensional (3D) scaffolds in tissue engineering technology. This leads to tissue necrosis that occurs in the central part of 3D scaffolds. To solve this issue, an effective cell seeding technique into the central part of 3D scaffolds is required. Chitosan has characteristics of biocompatibility, biodegradability, and low‐toxicity. In this study, we developed novel magnetic nanoparticles (MNs) coated with chitosan for enhancing cellular invasion using magnetic force. Cell‐invasion efficiency was enhanced by introducing our novel MNs into cells and by the presence of magnetic force. This invasion efficacy depends on the degree of magnetic force. Matrix metalloproteinases and adhesion molecules that were upregulated in response to the attached nanoparticles and exposure to a magnetic force may also play a crucial role in improving cell‐invasive ability in this system. This current system can efficiently enhance cell seeding into the depth of the scaffold, increase subsequent cell–cell interactions and shorten the period of cell proliferation. This system is thought to be useful in the development of cell‐based strategies for the repair or replacement of tissue and other novel therapies. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008