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Microarray‐based gene expression analysis of human osteoblasts in response to different biomaterials
Author(s) -
BombonatoPrado Karina F.,
Bellesini Larissa S.,
Junta Cristina M.,
Marques Márcia M.,
Passos Geraldo A.,
Rosa Adalberto L.
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31701
Subject(s) - osteoblast , biocompatibility , gene expression , materials science , microbiology and biotechnology , microarray , gene expression profiling , biomedical engineering , microarray analysis techniques , cell adhesion , tissue engineering , gene , adhesion , biology , medicine , in vitro , biochemistry , composite material , metallurgy
Several biomaterials have been widely used in bone regeneration/substitution procedures in orthopedic and oral surgery. However, how these biomaterials alter osteoblast gene expression is poorly understood. We therefore attempted to address this question by using cDNA microarray technique to identify genes that are differentially regulated in osteoblasts exposed to biomaterials comprehending the biocompatibility spectrum of bioactive (bioglass and hydroxyapatite), bioinert (Ti and stainless steel), and biotolerant (polymethylmethacrylate). By using a cDNA microarray containing 687 human IMAGE sequences, we identified in primary cultures of osteoblastic cells differentiated from the human bone marrow and exposed to these biomaterials, genes whose expression was significantly upregulated or downregulated. Among the differentially expressed genes we have found those involved with cell cycle regulation, cell differentiation and proliferation, apoptosis, cell adhesion, bone mineralization and skeletal development. These results can be relevant to a better understanding of the molecular mechanism underlying the behavior of osteoblasts in bone regenerative procedures. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009