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Control of the molecular degradation of hyaluronic acid hydrogels for tissue augmentation
Author(s) -
Oh Eun Ju,
Kang SunWoong,
Kim ByungSoo,
Jiang Ge,
Cho Il Hwan,
Hahn Sei Kwang
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31681
Subject(s) - self healing hydrogels , hyaluronic acid , hyaluronidase , solvent , materials science , ethanol , in vivo , polymer chemistry , swelling , degradation (telecommunications) , nuclear chemistry , chemistry , biochemistry , enzyme , biology , composite material , anatomy , telecommunications , microbiology and biotechnology , computer science
A novel protocol to control the molecular degradation of hyaluronic acid (HA) hydrogels was successfully developed for tissue augmentation applications. HA has a different conformational structure in water and organic solvent, and the carboxyl group of HA is known to be the recognition site of hyaluronidase and HA receptors. Based on these findings, HA was chemically modified by grafting adipic acid dihydrazide (ADH) to the carboxyl group of HA in the water to prepare HA‐ADH WATER and in the mixed solvent of water and ethanol to prepare degradation‐controlled HA‐ADH WATER/ETHANOL . Three kinds of HA hydrogels were prepared by the crosslinking of HA‐ADH WATER or HA‐ADH WATER/ETHANOL with bis(sulfosuccinimidyl) suberate, and by the crosslinking of HA‐OH with divinyl sulfone (DVS). In vitro and in vivo degradation tests showed that HA‐DVS hydrogels were degraded most rapidly, followed by HA‐ADH WATER hydrogels and HA‐ADH WATER/ETHANOL hydrogels. There was no adverse effect during and after in vivo degradation tests. All of the HA hydrogel samples appeared to be biocompatible, according to the histological analysis with hematoxylin–eosin and Alcian blue. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008