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Effect of borate glass composition on its conversion to hydroxyapatite and on the proliferation of MC3T3‐E1 cells
Author(s) -
Brown Roger F.,
Rahaman Mohamed N.,
Dwilewicz Agatha B.,
Huang Wenhai,
Day Delbert E.,
Li Yadong,
Bal B. Sonny
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31679
Subject(s) - materials science , boron , silicate glass , cell growth , incubation , bioactive glass , in vitro , cell culture , composition (language) , nuclear chemistry , chemical engineering , analytical chemistry (journal) , biophysics , chromatography , biochemistry , composite material , chemistry , organic chemistry , biology , linguistics , philosophy , engineering , genetics
Glasses containing varying amounts of B 2 O 3 were prepared by partially or fully replacing the SiO 2 in silicate 45S5 bioactive glass with B 2 O 3 . The effects of the B 2 O 3 content of the glass on its conversion to hydroxyapatite (HA) and on the proliferation of MC3T3‐E1 cells were investigated in vitro . Conversion of the glasses to HA in dilute (20 m M ) K 2 HPO 4 solution was monitored using weight loss and pH measurements. Proliferation of MC3T3‐E1 cells was determined qualitatively by assay of cell density at the glass interface after incubation for 1 day and 3 days, and quantitatively by fluorescent measurements of total DNA in cultures incubated for 4 days. Higher B 2 O 3 content of the glass increased the conversion rate to HA, but also resulted in a greater inhibition of cell proliferation under static culture conditions. For a given mass of glass in the culture medium, the inhibition of cell proliferation was alleviated by using glasses with lower B 2 O 3 content, by incubating the cell cultures under dynamic rather than static conditions, or by partially converting the glass to HA prior to cell culture. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009