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Subcutaneous transplantation of autologous oral mucosal epithelial cell sheets fabricated on temperature‐responsive culture dishes
Author(s) -
Obokata Haruko,
Yamato Masayuki,
Yang Joseph,
Nishida Kohji,
Tsuneda Satoshi,
Okano Teruo
Publication year - 2007
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31659
Subject(s) - transplantation , progenitor cell , oral mucosa , epithelium , stem cell , organ culture , immunology , pathology , microbiology and biotechnology , biology , medicine , in vitro , biochemistry
The oral mucosa is an attractive cell source for autologous transplantation in human patients who require regenerative therapies of various epithelia. However, the time‐course of cellular changes in transplanted oral mucosal epithelia at ectopic sites remains poorly understood. By applying a rat model, we analyzed phenotypic changes in oral mucosal epithelial cell sheets after harvest from temperature‐responsive culture dishes and subsequent autologous subcutaneous transplantation. We used monoclonal antibodies to identify epithelial‐specific cytokeratins 4, 10, 13, and 14, the stem/progenitor cell marker p63, and proliferating cell nuclear antigen, within the regenerated tissues. Transplanted oral mucosal epithelial cell sheets proliferated during the first week after grafting in conjunction with host inflammation, but then began to degenerate afterward with complete disappearance after 3 weeks. Our findings suggest that host subcutaneous tissues support proliferation and differentiation of the oral mucosal epithelial cell sheets, but are unable to promote maintenance of stem and progenitor cells and therefore cannot produce long‐term survivability. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008