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β‐TCP microporosity decreases the viability and osteoblast differentiation of human bone marrow stromal cells
Author(s) -
Isaac Juliane,
Hornez JeanChristophe,
Jian Ding,
Descamps Michel,
Hardouin Pierre,
Magne David
Publication year - 2008
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.31644
Subject(s) - materials science , stromal cell , alkaline phosphatase , osteocalcin , osteoblast , viability assay , calcium , bone marrow , microbiology and biotechnology , in vitro , immunology , biochemistry , chemistry , biology , cancer research , metallurgy , enzyme
Association of osteoprogenitor cells to calcium phosphate ceramics is currently under intense investigation, for its considered ability to induce bone formation and therefore to allow the successful repair of large bone defects. However, if the first experimental and clinical studies provided promising results, lack of new bone formation has been reported in a large number of animal experiments. In this context and since it has been reported that in some conditions, calcium phosphate ceramic microstructure induces ectopic bone formation, we investigated the effects of ceramic microporosity on the behavior of osteoprogenitor cells for the development of hybrid materials. Human bone marrow stromal cells (BMSCs) were seeded on β‐tricalcium phosphate (TCP) ceramics with 0, 25, or 45% microporosity and cell adhesion, viability, and osteoblastic differentiation have been studied for three weeks. Cell counts, measurement of MTS conversion, and LDH activity indicated that microporosity decreased the viability of BMSCs in a time and rate‐dependent manner. In addition, microporosity inhibited osteoblastic differentiation as compared with nonmicroporous ceramics, as revealed by decreased alkaline phosphatase activity and osteocalcin secretion. Results of this in vitro study therefore highlight a negative role for β‐TCP microporosity in the behavior of human osteoprogenitor cells. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008